Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation.

ABSTRACT: Wide-field fluorescence imaging is an indispensable tool for studying large-scale biodynamics. Limited space-bandwidth product and strong light diffusion make conventional implementations incapable of high-resolution mapping of fluorescence biodistribution in three dimensions. We introduce a volumetric wide-field fluorescence microscopy based on optical astigmatism combined with fluorescence source localization, covering 5.6×5.6×0.6 mm³ imaging volume. Two alternative configurations are proposed exploiting multifocal illumination or sparse localization of point emitters, which are herein seamlessly integrated in one system. We demonstrate real-time volumetric mapping of the murine cortical microcirculation at capillary resolution without employing cranial windows, thus simultane

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC9797555 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature