Hyperthermostable recombinant human heteropolymer ferritin derived from a novel plasmid design.

ABSTRACT: Mammalian ferritins are predominantly heteropolymeric species consisting of 2 structurally similar, but functionally and genetically distinct subunit types, called H (Heavy) and L (Light). The two subunits co-assemble in different H and L ratios to form 24-mer shell-like protein nanocages where thousands of iron atoms can be mineralized inside a hollow cavity. Here, we use differential scanning calorimetry (DSC) to study ferritin stability and understand how various combinations of H and L subunits confer aspects of protein structure-function relationships. Using a recently engineered plasmid design that enables the synthesis of complex ferritin nanostructures with specific H to L subunit ratios, we show that homopolymer L and heteropolymer L-rich ferritins have a remarkable hyperthermosta

SUBMITTER: Srivastava AK 

PROVIDER: S-EPMC9798250 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature