Metagenomic sequencing of the bronchoalveolar lavage extracellular virome and cellular transcriptome of sarcoidosis patients does not detect rubella virus.

ABSTRACT:

Background

  Sarcoidosis is a multisystem granulomatous inflammatory disease of unclear etiology that involves the lung, skin and other organs, with an unknown antigenic trigger. Recently, evidence has been found in both immune deficient and immune competent patients for rubella virus in cutaneous granulomas. These granulomatous lesions share overlapping features with cutaneous sarcoidosis, raising the question of rubella virus in sarcoidosis.

Objective

To investigate the presence of rubella virus in sarcoidosis lung samples.

Methods

We employed metagenomic sequencing to interrogate extracellular virome preparations and cellular transcriptomes from bronchoalveolar lavage (BAL) of 209 sarcoidosis patients for rubella virus sequences.

Results

We found no evidence

SUBMITTER: Keeler EL 

PROVIDER: S-EPMC9798337 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature