Intracellular Dynamics of Extracellular Vesicles by Segmented Trajectory Analysis.

ABSTRACT: The analysis of nanoparticle (NP) dynamics in live cell studies by video tracking provides detailed information on their interactions and trafficking in the cells. Although the video analysis is not yet routinely used in NP studies, the equipment suitable for the experiments is already available in most laboratories. Here, we compare trajectory patterns, diffusion coefficients, and particle velocities of NPs in A549 cells with a rather simple experimental setup consisting of a fluorescence microscope and openly available trajectory analysis software. The studied NPs include commercial fluorescent polymeric particles and two subpopulations of PC-3 cell-derived extracellular vesicles (EVs). As bioderived natural nanoparticles, the fluorescence intensities of the EVs limited the recording spe

SUBMITTER: Rautaniemi K 

PROVIDER: S-EPMC9798377 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature