High-efficiency production of 5-hydroxyectoine using metabolically engineered Corynebacterium glutamicum.

ABSTRACT:

Background

Extremolytes enable microbes to withstand even the most extreme conditions in nature. Due to their unique protective properties, the small organic molecules, more and more, become high-value active ingredients for the cosmetics and the pharmaceutical industries. While ectoine, the industrial extremolyte flagship, has been successfully commercialized before, an economically viable route to its highly interesting derivative 5-hydroxyectoine (hydroxyectoine) is not existing.

Results

Here, we demonstrate high-level hydroxyectoine production, using metabolically engineered strains of C. glutamicum that express a codon-optimized, heterologous ectD gene, encoding for ectoine hydroxylase, to convert supplemented ectoine in the presence of sucrose as growth substrate into

SUBMITTER: Jungmann L 

PROVIDER: S-EPMC9798599 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature