Tailor-made alkaliphilic and thermostable fungal laccases for industrial wood processing.

ABSTRACT:

Background

During the kraft process to obtain cellulosic pulp from wood, most of the lignin is removed by high-temperature alkaline cooking, released in the black liquors and usually incinerated for energy. However, kraft lignins are a valuable source of phenolic compounds that can be valorized in new bio-based products. The aim of this work is to develop laccases capable of working under the extreme conditions of high temperature and pH, typical of the industrial conversion of wood into kraft pulp and fibreboard, in order to provide extremophilic biocatalysts for depolymerising kraft lignin, and enzyme-assisted technologies for kraft pulp and fibreboard production.

Results

Through systematic enzyme engineering, combining enzyme-directed evolution and rational design, we cha

SUBMITTER: Rodriguez-Escribano D 

PROVIDER: S-EPMC9798632 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature