A novel fusion protein candidate for the serodiagnosis of Mycoplasma agalactiae infection.

ABSTRACT:

Background

The aim of current study was to construct, express, purify and immunogenicity evaluate of a novel recombinant fusion protein including Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of lipoprotein P80 of Mycoplasma agalactiae. Using bioinformatics tools, antigenicity and physiochemical properties of fused protein were assessed.

Material and methods

The recombinant fusion protein of GST-PDHB-P80 were expressed in pGEX4T-1 and purified then verified by Western blot assay. The purified protein was successfully used for immunization of mice. 30 female BALB/c mice were divided into three groups (10 mice per each group) injected with GST-PDHB-P80, inactivated bacteria vaccine and PBS as negative control, separately.

Results

Western blot anal

SUBMITTER: Akbarzadeh-Niaki M 

PROVIDER: S-EPMC9798644 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature