Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid.

ABSTRACT:

Background

Poly γ-glutamic acid (γ-PGA) is a promising biopolymer for various applications. For glutamic acid-independent strains, the titer of γ-PGA is too low to meet the industrial demand. In this study, we isolated a novel γ-PGA-producing strain, Bacillus tequilensis BL01, and multiple genetic engineering strategies were implemented to improve γ-PGA production.

Results

First, the one-factor-at-a-time method was used to investigate the influence of carbon and nitrogen sources and temperature on γ-PGA production. The optimal sources of carbon and nitrogen were sucrose and (NH₄)₂SO₄ at 37 °C, respectively. Second, the sucA, gudB, pgdS, and ggt genes were knocked out simultaneously, which increased the titer of γ-PGA by 1.75 times. Then, the

SUBMITTER: Wang D 

PROVIDER: S-EPMC9798646 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature