Maintenance of methylation profile in imprinting control regions in human induced pluripotent stem cells.

ABSTRACT:

Background

Parental imprinting is an epigenetic mechanism that leads to monoallelic expression of a subset of genes depending on their parental origin. Imprinting disorders (IDs), caused by disturbances of imprinted genes, are a set of rare congenital diseases that mainly affect growth, metabolism and development. To date, there is no accurate model to study the physiopathology of IDs or test therapeutic strategies. Human induced pluripotent stem cells (iPSCs) are a promising cellular approach to model human diseases and complex genetic disorders. However, aberrant hypermethylation of imprinting control regions (ICRs) may appear during the reprogramming process and subsequent culture of iPSCs. Therefore, we tested various conditions of reprogramming and culture of iPSCs and perform

SUBMITTER: Pham A 

PROVIDER: S-EPMC9798676 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature