An Extended DNA Binding Domain of the Estrogen Receptor Alpha Directly Interacts with RNAs <i>in Vitro</i>.

ABSTRACT: Estrogen receptor alpha (ERα) is a ligand-responsive transcription factor critical for sex determination and development. Recent reports challenge the canonical view of ERα function by suggesting an activity beyond binding dsDNA at estrogen-responsive promotor elements: association with RNAs in vivo. Whether these interactions are direct or indirect remains unknown, which limits the ability to understand the extent, specificity, and biological role of ERα-RNA binding. Here we demonstrate that an extended DNA-binding domain of ERα directly binds a wide range of RNAs in vitro with structural specificity. ERα binds RNAs that adopt a range of hairpin-derived structures independent of sequence, while interacting poorly with single- and double-stranded RNA. RNA affinities are only

SUBMITTER: Steiner HR 

PROVIDER: S-EPMC9798703 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature