Probing RNA Conformations Using a Polymer-Electrolyte Solid-State Nanopore.

ABSTRACT: Nanopore systems have emerged as a leading platform for the analysis of biomolecular complexes with single-molecule resolution. The conformation of biomolecules, such as RNA, is highly dependent on the electrolyte composition, but solid-state nanopore systems often require high salt concentration to operate, precluding analysis of macromolecular conformations under physiologically relevant conditions. Here, we report the implementation of a polymer-electrolyte solid-state nanopore system based on alkali metal halide salts dissolved in 50% w/v poly(ethylene) glycol (PEG) to augment the performance of our system. We show that polymer-electrolyte bath governs the translocation dynamics of the analyte which correlates with the physical properties of the salt used in the bath. This allowed us t

SUBMITTER: Chau C 

PROVIDER: S-EPMC9798860 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature