Cross-contamination risk and decontamination during changeover after cell-product processing

ABSTRACT:

Introduction

During changeover in cell-product processing, it is essential to minimize cross-contamination risks. These risks differ depending on the patient from whom the cells were derived. Human error during manual cell-product processing increases the contamination risk in biosafety cabinets. Here, we evaluate the risk of cross-contamination during manual cell-processing to develop an evidence-based changeover method for biosafety cabinets.

Methods

Contaminant coverage was analyzed during simulated medium preparation, cell seeding, and waste liquid decanting by seven operators, classified by skill. Environmental bacteria were surveyed at four participating facilities. Finally, we assessed the effect of conventional UV irradiation in biosafety cabinets on bacteria and

SUBMITTER: Mizuno M 

PROVIDER: S-EPMC9800260 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature