The role of mitochondria in pharmacological ascorbate-induced toxicity.

ABSTRACT: At pharmacological levels, ascorbate (P-AscH^-) acts as a pro-oxidant by generating H₂O₂, depleting ATP in sensitive cells leading to cell death. The aim of this study was to determine the role of ATP production by oxidative phosphorylation or glycolysis in mechanisms of resistance to P-AscH^-induced cell death. Pancreatic cancer cells were used to generate ρ⁰ cells by mitochondrial overexpression of the Y147A mutant uracil-N-glycosylase or Herpes Simplex Virus protein. The ρ⁰ phenotype was confirmed by probing for mitochondrial DNA, mitochondrial DNA-encoded cytochrome c oxidase subunit 2, and monitoring the rate of oxygen consumption. In ρ⁰ cells, glycolysis accounted for 100% of ATP production as there was no mit

SUBMITTER: Du J 

PROVIDER: S-EPMC9800562 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature