Application of mutational profiling: New functional analyses reveal the tRNA recognition mechanism of tRNA m¹A22 methyltransferase.

ABSTRACT: Transfer RNAs undergo diverse posttranscriptional modifications to regulate a myriad of cellular events including translation, stress response, and viral replication. These posttranscriptional modifications are synthesized by site-specific modification enzymes. Recent RNA-seq techniques have revealed multiple features of tRNA such as tRNA abundance, tRNA modification, and tRNA structure. Here, we adapt a tRNA-sequencing technique and design a new functional analysis where we perform mutational profiling of tRNA modifications to gain mechanistic insights into how tRNA modification enzymes recognize substrate tRNA. Profiling of Geobacillus stearothermophilus tRNAs and protein orthology analysis predict the existence of natural modifications in 44 tRNA molecular species of G. stearothermophil

SUBMITTER: Yamagami R 

PROVIDER: S-EPMC9801127 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature