Cocaine's effects on the reactivity of the medial prefrontal cortex to ventral tegmental area stimulation: optical imaging study in mice.

ABSTRACT:

Background and aims

The prefrontal cortex (PFC) is modulated by dopaminergic and glutamatergic neurons that project from the ventral tegmental area (VTA) and disruption of this modulation might facilitate impulsive behaviors during cocaine intoxication. Here, we assessed the effects of acute cocaine (30 mg/kg, i.p.) on the reactivity of the PFC to VTA stimulation.

Methods

Using a genetically encoded calcium indicator (GCaMP6f), we optically imaged the neuronal Ca²⁺ reactance in medial PFC (mPFC) in response to 'tonic-like' (5 Hz) and 'phasic-like' (50 Hz) electrical VTA stimulation. The high temporal and spatial resolutions of our optical system allowed us to capture single Ca²⁺ neuronal transients from individual stimuli with 'tonic-like' stimulation a

SUBMITTER: Park K 

PROVIDER: S-EPMC9801493 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature