DNA methylation in human gastric epithelial cells defines regional identity without restricting lineage plasticity.

ABSTRACT:

Background

Epigenetic modifications in mammalian DNA are commonly manifested by DNA methylation. In the stomach, altered DNA methylation patterns have been observed following chronic Helicobacter pylori infections and in gastric cancer. In the context of epigenetic regulation, the regional nature of the stomach has been rarely considered in detail.

Results

Here, we establish gastric mucosa derived primary cell cultures as a reliable source of native human epithelium. We describe the DNA methylation landscape across the phenotypically different regions of the healthy human stomach, i.e., antrum, corpus, fundus together with the corresponding transcriptomes. We show that stable regional DNA methylation differences translate to a limited extent into regulation of the transcript

SUBMITTER: Fritsche K 

PROVIDER: S-EPMC9801550 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature