High-quality genome-scale metabolic network reconstruction of probiotic bacterium Escherichia coli Nissle 1917.

ABSTRACT:

Background

Escherichia coli Nissle 1917 (EcN) is a probiotic bacterium used to treat various gastrointestinal diseases. EcN is increasingly being used as a chassis for the engineering of advanced microbiome therapeutics. To aid in future engineering efforts, our aim was to construct an updated metabolic model of EcN with extended secondary metabolite representation.

Results

An updated high-quality genome-scale metabolic model of EcN, iHM1533, was developed based on comparison with 55 E. coli/Shigella reference GEMs and manual curation, including expanded secondary metabolite pathways (enterobactin, salmochelins, aerobactin, yersiniabactin, and colibactin). The model was validated and improved using phenotype microarray data, resulting in an 82.3% accuracy in predicting growt

SUBMITTER: van 't Hof M 

PROVIDER: S-EPMC9801561 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature