Three-dimensional nanoscale analysis of light-dependent organelle changes in Arabidopsis mesophyll cells.

ABSTRACT: Different organelles function coordinately in numerous intracellular processes. Photorespiration incidental to photosynthetic carbon fixation is organized across three subcellular compartments: chloroplasts, peroxisomes, and mitochondria. Under light conditions, these three organelles often form a ternary organellar complex in close proximity, suggesting a connection with metabolism during photorespiration. However, due to the heterogeneity of intercellular organelle localization and morphology, organelles' responses to changes in the external environment remain poorly understood. Here, we used array tomography by field emission scanning electron microscopy to image organelles inside the whole plant cell at nanometer resolution, generating a three-dimensional (3D) spatial map of the light-

SUBMITTER: Midorikawa K 

PROVIDER: S-EPMC9802074 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature