Dataset Information

Single-cell sequencing reveals novel cellular heterogeneity in uterine leiomyomas.

ABSTRACT:

Study question

What are the cellular composition and single-cell transcriptomic differences between myometrium and leiomyomas as defined by single-cell RNA sequencing?

Summary answer

We discovered cellular heterogeneity in smooth muscle cells (SMCs), fibroblast and endothelial cell populations in both myometrium and leiomyoma tissues.

What is known already

Previous studies have shown the presence of SMCs, fibroblasts, endothelial cells and immune cells in myometrium and leiomyomas. However, there is no information on the cellular heterogeneity in these tissues and the transcriptomic differences at the single-cell level between these tissues.

Study design, size, duration

We collected five leiomyoma and five myometrium samples from a total of eight patients undergoing hysterectomy. We then performed single-cell RNA sequencing to generate a cell atlas for both tissues. We utilized our single-cell sequencing data to define cell types, compare cell types by tissue type (leiomyoma versus myometrium) and determine the transcriptional changes at a single-cell resolution between leiomyomas and myometrium. Additionally, we performed MED12-variant analysis at the single-cell level to determine the genotype heterogeneity within leiomyomas.

Participants/materials, setting, methods

We collected five MED12-variant positive leiomyomas and five myometrium samples from a total of eight patients. We then performed single-cell RNA sequencing on freshly isolated single-cell preparations. Histopathological assessment confirmed the identity of the samples. Sanger sequencing was performed to confirm the presence of the MED12 variant in leiomyomas.

Main results and role of chance

Our data revealed previously unknown heterogeneity in the SMC, fibroblast cell and endothelial cell populations of myometrium and leiomyomas. We discovered the presence of two different lymphatic endothelial cell populations specific to uterine leiomyomas. We showed that both myometrium and MED12-variant leiomyomas are relatively similar in cellular composition but differ in cellular transcriptomic profiles. We found that fibroblasts influence the leiomyoma microenvironment through their interactions with endothelial cells, immune cells and SMCs. Variant analysis at the single-cell level revealed the presence of both MED12 variants as well as the wild-type MED12 allele in SMCs of leiomyomatous tissue. These results indicate genotype heterogeneity of cellular composition within leiomyomas.

Large scale data

The datasets are available in the NCBI Gene Expression Omnibus (GEO) using GSE162122.

Limitations, reasons for caution

Our study focused on MED12-variant positive leiomyomas for single-cell RNA sequencing analyses. Leiomyomas carrying other genetic rearrangements may differ in their cellular composition and transcriptomic profiles.

Wider implications for the findings

Our study provides a cellular atlas for myometrium and MED12-variant positive leiomyomas as defined by single-cell RNA sequencing. Our analysis provides significant insight into the differences between myometrium and leiomyomas at the single-cell level and reveals hitherto unknown genetic heterogeneity in multiple cell types within human leiomyomas. Our results will be important for future studies into the origin and growth of human leiomyomas.

Study funding/competing interest(s)

This work was supported by funding from the National Institute of Child Health and Human Development (HD098580 and HD088629). The authors declare no competing interests.

SUBMITTER: Goad J

PROVIDER: S-EPMC9802286 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Publications

Single-cell sequencing reveals novel cellular heterogeneity in uterine leiomyomas.

Goad Jyoti J Rudolph Joshua J Zandigohar Mehrdad M Tae Matthew M Dai Yang Y Wei Jian-Jun JJ Bulun Serdar E SE Chakravarti Debabrata D Rajkovic Aleksandar A

Human reproduction (Oxford, England) 20220901 10

<h4>Study question</h4>What are the cellular composition and single-cell transcriptomic differences between myometrium and leiomyomas as defined by single-cell RNA sequencing?<h4>Summary answer</h4>We discovered cellular heterogeneity in smooth muscle cells (SMCs), fibroblast and endothelial cell populations in both myometrium and leiomyoma tissues.<h4>What is known already</h4>Previous studies have shown the presence of SMCs, fibroblasts, endothelial cells and immune cells in myometrium and lei ...[more]

PMID: 36001050

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Dataset Information

Single-cell sequencing reveals novel cellular heterogeneity in uterine leiomyomas.

Study question

Summary answer

What is known already

Study design, size, duration

Participants/materials, setting, methods

Main results and role of chance

Large scale data

Limitations, reasons for caution

Wider implications for the findings

Study funding/competing interest(s)

Publications

Single-cell sequencing reveals novel cellular heterogeneity in uterine leiomyomas.

Similar Datasets

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