CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

ABSTRACT: In recent years, Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) has emerged as a flexible method that enables semi-automated volume ultrastructural imaging. We present a toolset for adherent cells that enables tracking and finding cells, previously identified in light microscopy (LM), in the FIB-SEM, along with the automatic acquisition of high-resolution volume datasets. We detect the underlying grid pattern in both modalities (LM and EM), to identify common reference points. A combination of computer vision techniques enables complete automation of the workflow. This includes setting the coincidence point of both ion and electron beams, automated evaluation of the image quality and constantly tracking the sample position with the microscope's field of view reducing or even elimi

SUBMITTER: Serra Lleti JM 

PROVIDER: S-EPMC9802685 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature