M⁶A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site.

ABSTRACT: Alternative splicing of messenger RNAs is associated with the evolution of developmentally complex eukaryotes. Splicing is mediated by the spliceosome, and docking of the pre-mRNA 5' splice site into the spliceosome active site depends upon pairing with the conserved ACAGA sequence of U6 snRNA. In some species, including humans, the central adenosine of the ACAGA box is modified by N⁶ methylation, but the role of this m⁶A modification is poorly understood. Here, we show that m⁶A modified U6 snRNA determines the accuracy and efficiency of splicing. We reveal that the conserved methyltransferase, FIONA1, is required for Arabidopsis U6 snRNA m⁶A modification. Arabidopsis fio1 mutants show disrupted patterns of splicing th

SUBMITTER: Parker MT 

PROVIDER: S-EPMC9803359 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature