MC profiling: a novel approach to analyze DNA methylation heterogeneity in genome-wide bisulfite sequencing data.

ABSTRACT: DNA methylation is an epigenetic mark implicated in crucial biological processes. Most of the knowledge about DNA methylation is based on bulk experiments, in which DNA methylation of genomic regions is reported as average methylation. However, average methylation does not inform on how methylated cytosines are distributed in each single DNA molecule. Here, we propose Methylation Class (MC) profiling as a genome-wide approach to the study of DNA methylation heterogeneity from bulk bisulfite sequencing experiments. The proposed approach is built on the concept of MCs, groups of DNA molecules sharing the same number of methylated cytosines. The relative abundances of MCs from sequencing reads incorporates the information on the average methylation, and directly informs on the methylation lev

SUBMITTER: De Riso G 

PROVIDER: S-EPMC9803872 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature