Optimization of cryo-electron microscopy for quantitative analysis of lipid bilayers.

ABSTRACT: Cryogenic electron microscopy (cryo-EM) is among the most powerful tools available for interrogating nanoscale structure of biological materials. We recently showed that cryo-EM can be used to measure the bilayer thickness of lipid vesicles and biological membranes with subangstrom precision, resulting in the direct visualization of nanoscopic domains of different thickness in multicomponent lipid mixtures and giant plasma membrane vesicles. Despite the great potential of cryo-EM for revealing the lateral organization of biomembranes, a large parameter space of experimental conditions remains to be optimized. Here, we systematically investigate the influence of instrument parameters and image postprocessing steps on the ability to accurately measure bilayer thickness and discriminate regio

SUBMITTER: Heberle FA 

PROVIDER: S-EPMC9804012 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature