Disruption of Mitochondrial-associated ER membranes by HIV-1 tat protein contributes to premature brain aging.

ABSTRACT:

Introduction

Mitochondrial-associated ER membranes (MAMs) control many cellular functions, including calcium and lipid exchange, intracellular trafficking, and mitochondrial biogenesis. The disruption of these functions contributes to neurocognitive disorders, such as spatial memory impairment and premature brain aging. Using neuronal cells, we demonstrated that HIV-1 Tat protein deregulates the mitochondria.

Methods& results

To determine the mechanisms, we used a neuronal cell line and showed that Tat-induced changes in expression and interactions of both MAM-associated proteins and MAM tethering proteins. The addition of HIV-1 Tat protein alters expression levels of PTPIP51 and VAPB proteins in the MAM fraction but not the whole cell. Phosphorylation of PTPIP51 protein reg

SUBMITTER: Arjona SP 

PROVIDER: S-EPMC9804058 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature