A novel fully-automated method to measure steroids in serum by liquid chromatography-tandem mass spectrometry.

ABSTRACT:

Background

Steroids play a key role in numerous physiological processes. Steroid determination is a useful tool to explore various endocrine diseases. Because of its specificity, mass spectrometry is considered to be a reference method for the determination of steroids in serum compared to radioimmunoassay. This technology could progress towards more automation for the optimal organization of clinical laboratories and ultimately for the benefit of patients.

Methods

A fully automated ultra-high-performance liquid chromatography-tandem mass spectrometry method was developed and fully validated to determine five steroids in serum. Sample preparation was based on protein precipitation with filtration followed by online solid phase extraction. Chromatographic separation was perfo

SUBMITTER: Fraissinet F 

PROVIDER: S-EPMC9804132 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature