Improving cooperativity of transcription activators by oligomerization domains in mammalian cells.

ABSTRACT: Cooperative activation is critical for the applications of synthetic biology in mammalian cells. In this study, we have developed cooperative transcription factor by fusing oligomerization domain in mammalian cells. Firstly, we demonstrated that two oligomerized domains (CI434 and CI) successfully improved transcription factor cooperativity in bacterial cells but failed to increase cooperativity in mammalian cells, possibly because the additional mammalian activation domain disrupted their oligomerization capability. Therefore, we chose a different type of oligomerized domain (CarH_C), whose ability to oligomerize is not dependent on its C-terminal domains, to fuse with a transcription factor (RpaR) and activation domain (VTR3), forming a potential cooperative transcription activ

SUBMITTER: Chen X 

PROVIDER: S-EPMC9804245 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature