Development of versatile affinity-based system for one step purification process: Case of Group A Streptococcus vaccine.

ABSTRACT: Affinity capture is one of the most attractive strategies for simplifying downstream processing. Although it is a key mainstream approach for antibody purification, the same is not true for other biologics such as vaccines, mainly due to the lack of suitable affinity material. In this study, a novel custom affinity system is introduced permitting widespread adoption of affinity capture for the purification of biologics beyond antibodies. This is illustrated here by the development of a one-step purification process of a mutant form of streptolysin O (SLO), a vaccine candidate against Streptococcus pyogenes infection. The system consists of the association of custom ligands based on the Nanofitin protein scaffold, with Eshmuno® industry-grade chromatography medium. The Nanofitins were selec

SUBMITTER: Chevrel A 

PROVIDER: S-EPMC9804325 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature