Metabolic phenotyping of acquired ampicillin resistance using microbial volatiles from Escherichia coli cultures.

ABSTRACT:

Aims

This study sought to assess the volatile organic compound (VOC) profiles of ampicillin-resistant and -susceptible Escherichia coli to evaluate whether VOC analysis may be utilized to identify resistant phenotypes.

Methods and results

An E. coli BL21 (DE3) strain and its pET16b plasmid transformed ampicillin-resistant counterpart were cultured for 6 h in drug-free, low- and high-concentrations of ampicillin. Headspace analysis was undertaken using thermal desorption-gas chromatography-mass spectrometry. Results revealed distinct VOC profiles with ampicillin-resistant bacteria distinguishable from their susceptible counterparts using as few as six compounds. A minimum of 30 compounds (fold change >2, p ≤ 0.05) were differentially expressed between the strains across all s

SUBMITTER: Dixon B 

PROVIDER: S-EPMC9804386 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature