An in vitro and ex vivo wound infection model to test topical and systemic treatment with antibiotics.

ABSTRACT:

Aims

This study aimed to develop a wound infection model that could be used to test antibiotic-loaded electrospun matrices for the topical treatment of infected skin and compare the effectiveness of this treatment to systemically applied antibiotics.

Methods and results

3D-printed flow chambers were made in which Staphylococcus aureus biofilms were grown either on a polycarbonate membrane or explanted porcine skin. The biofilms were then treated either topically, by placing antibiotic-loaded electrospun matrices on top of the biofilms, or systemically by the addition of antibiotics in the growth medium that flowed underneath the membrane or skin. The medium that was used was either a rich medium or an artificial wound fluid. The results showed that microbial viability in the

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC9804477 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature