A reactive oxygen species Ca²⁺ signalling pathway identified from a chemical screen for modifiers of sugar-activated circadian gene expression.

ABSTRACT: Sugars are essential metabolites for energy and anabolism that can also act as signals to regulate plant physiology and development. Experimental tools to disrupt major sugar signalling pathways are limited. We performed a chemical screen for modifiers of activation of circadian gene expression by sugars to discover pharmacological tools to investigate and manipulate plant sugar signalling. Using a library of commercially available bioactive compounds, we identified 75 confident hits that modified the response of a circadian luciferase reporter to sucrose in dark-adapted Arabidopsis thaliana seedlings. We validated the transcriptional effect on a subset of the hits and measured their effects on a range of sugar-dependent phenotypes for 13 of these chemicals. Chemicals were identified that

SUBMITTER: Li X 

PROVIDER: S-EPMC9804775 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature