Effects of cell seeding technique and cell density on BMP-2 production in transduced human mesenchymal stem cells.

ABSTRACT: Small animal models have demonstrated the efficacy of ex vivo regional gene therapy using scaffolds loaded with BMP-2-expressing mesenchymal stem cells (MSCs). Prior to clinical translation, optimization of seeding techniques of the transduced cells will be important to minimize time and resource expenditure, while maximizing cell delivery and BMP-2 production. No prior studies have investigated cell-seeding techniques in the setting of transduced cells for gene therapy applications. Using BMP-2-expressing transduced adipose-derived MSCs and a porous ceramic scaffold, this study compared previously described static and dynamic seeding techniques with respect to cell seeding efficiency, uniformity of cell distribution, and in vitro BMP-2 production. Static and negative pressure seeding tech

SUBMITTER: Collon K 

PROVIDER: S-EPMC9804863 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature