Suppression of myeloid PFKFB3-driven glycolysis protects mice from choroidal neovascularization.

ABSTRACT:

Background and purpose

Pathological angiogenesis is a major cause of irreversible blindness in individuals with neovascular age-related macular degeneration (nAMD). Macrophages and microglia (MΦ) contribute to aberrant ocular angiogenesis. However, the role of glucose metabolism of MΦ in nAMD is still undefined. Here, we have investigated the involvement of glycolysis, driven by the kinase/phosphatase PFKFB3, in the development of choroidal neovascularization (CNV).

Experimental approach

CNV was induced in mice with laser photocoagulation. Choroid/retinal pigment epithelium (RPE) complexes and MΦ were isolated for analysis by qRT-PCR, western blot, flow cytometry, immunostaining, metabolic measurements and angiogenesis assays.

Key results

MΦ accumulated within the CNV

SUBMITTER: Liu Z 

PROVIDER: S-EPMC9804921 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature