Insulin expression in β cells is reduced within islets before islet loss in diabetic cats.

ABSTRACT:

Objectives

Diabetes mellitus is a common condition that requires intensive treatment and markedly impacts the welfare of affected cats. The aim of this study was to identify diabetes mellitus-associated perturbations in the feline pancreatic islet microenvironment. The utility of "clear, unobstructed brain/body imaging cocktails and computational analysis" (CUBIC) for three-dimensional pancreatic analysis was investigated.

Methods

Formalin-fixed paraffin-embedded tissues from cats with diabetes mellitus, or control cats without pancreatic pathology, were retrospectively identified. Immunohistochemistry for synaptophysin and ionised calcium binding adaptor molecule 1, and immunofluorescence for insulin and synaptophysin, were used to assess changes in islets. An image analysi

SUBMITTER: Bergomi V 

PROVIDER: S-EPMC9804985 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature