Enhancing the Peroxygenase Activity of a Cofactor-Independent Peroxyzyme by Directed Evolution Enabling Gram-Scale Epoxide Synthesis.

ABSTRACT: Peroxygenases selectively incorporate oxygen into organic molecules making use of the environmentally friendly oxidant H₂ O₂ with water being the sole by-product. These biocatalysts can provide 'green' routes for the synthesis of enantioenriched epoxides, which are fundamental intermediates in the production of pharmaceuticals. The peroxyzyme 4-oxalocrotonate tautomerase (4-OT), catalysing the epoxidation of a variety of α,β-unsaturated aldehydes with H₂ O₂ , is outstanding because of its independence from any cost-intensive cofactor. However, its low-level peroxygenase activity and the decrease in the enantiomeric excess of the corresponding α,β-epoxy-aldehydes under preparative-scale conditions is limiting the potential of 4-OT. Herein we repor

SUBMITTER: Sigmund MC 

PROVIDER: S-EPMC9804992 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature