Modular co-culture engineering of Yarrowia lipolytica for amorphadiene biosynthesis.

ABSTRACT: Amorphadiene is the precursor to synthesize the antimalarial drug artemisinin. The production of amorphadiene and artemisinin from metabolically engineered microbes may provide an alternate to plant secondary metabolite extraction. Microbial consortia can offer division of labor, and microbial co-culture system can be leveraged to achieve cost-efficient production of natural products. Using a co-culture system of Y. lipolytica Po1f and Po1g strains, subcellular localization of ADS gene (encoding amorphadiene synthase) into the endoplasmic reticulum, co-utilization of mixed carbon source, and enlargement of the endoplasmic reticulum (ER) surface area, we were able to significantly improve amorphadiene production in this work. Using Po1g/PPtM and Po1f/AaADSER_x3/iGFMPDU strains and

SUBMITTER: Marsafari M 

PROVIDER: S-EPMC9805133 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature