Separation and characterization of bovine milk proteins by capillary electrophoresis-mass spectrometry.

ABSTRACT: Protein profiling of major bovine milk proteins (i.e., whey and casein proteins) is of great interest in food science and technology. This complex set of protein proteoforms may vary with breed, genetics, lactation stage, health, and nutritional status of the animal. Current routine methods for bovine milk protein profiling at the intact level are typically based on capillary electrophoresis-ultraviolet, which does not allow confirming unequivocally the identity of the separated proteins. As an alternative, in this study, we describe for the first time a novel and simple capillary electrophoresis-mass spectrometry method in positive electrospray ionization mode. Under the optimized conditions, capillary electrophoresis-mass spectrometry allowed the separation and identification at the inta

SUBMITTER: Ghafoori Z 

PROVIDER: S-EPMC9805173 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature