A Novel Murine Model of Endothelial Keratoplasty.

ABSTRACT:

Purpose

The purpose of this study was to establish a murine model of endothelial keratoplasty.

Methods

Endothelial keratoplasty (EK) was performed using C57BL/6 donor and BALB/c recipient mice. The central endothelium and Descemet membrane were removed from the recipient cornea, and a 1.5-mm posterior lamellar donor graft was made adherent to the recipient cornea with a small amount of viscoelastic. Mice were followed through slitlamp microscopy postoperatively, and OCT was used to assess the cornea and anterior chamber and measure central corneal thickness. Histology and immunohistochemistry were performed to confirm graft adherence and endothelial cell morphology.

Results

Successfully attached EK grafts were visualized in all transplanted animals. Histology and immu

SUBMITTER: Nakagawa H 

PROVIDER: S-EPMC9805546 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature