Transcriptional profiling from whole embryos to single neuroblast lineages in Drosophila.

ABSTRACT: Embryonic development results in the production of distinct tissue types, and different cell types within each tissue. A major goal of developmental biology is to uncover the "parts list" of cell types that comprise each organ. Here we perform single cell RNA sequencing (scRNA-seq) of the Drosophila embryo to identify the genes that characterize different cell and tissue types during development. We assay three different timepoints, revealing a coordinated change in gene expression within each tissue. Interestingly, we find that the elav and Mhc genes, whose protein products are widely used as markers for neurons and muscles, respectively, show broad pan-embryonic expression, indicating the importance of post-transcriptional regulation. We next focus on the central nervous system (CNS), wh

SUBMITTER: Seroka A 

PROVIDER: S-EPMC9805786 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature