Human Lung Organoid Culture in Alginate With and Without Matrigel to Model Development and Disease.

ABSTRACT: Human lung organoids (HLOs) are enabling the study of human lung development and disease by modeling native organ tissue structure, cellular composition, and cellular organization. In this report, we demonstrate that HLOs derived from human pluripotent stem cells cultured in alginate, a fully defined nonanimal product substrate, exhibit enhanced cellular differentiation compared with HLOs cultured in the commercially available Matrigel. More specifically, we observed an earlier onset and increase in the number of multiciliated cells, along with mucus producing MUC5AC⁺ goblet-like cells that were not observed in HLOs cultured in Matrigel. The epithelium in alginate-grown HLOs was organized in a pseudostratified epithelium with airway basal cells lining the basal lamina, but with

SUBMITTER: Dye BR 

PROVIDER: S-EPMC9805886 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature