NLRP3 activation contributes to endothelin-1-induced erectile dysfunction.

ABSTRACT: In the present study, we hypothesized that endothelin (ET) receptors (ET_A and ET_B ) stimulation, through increased calcium and ROS formation, leads to Nucleotide Oligomerization Domain-Like Receptor Family, Pyrin Domain Containing 3 (NLRP3) activation. Intracavernosal pressure (ICP/MAP) was measured in C57BL/6 (WT) mice. Functional and immunoblotting assays were performed in corpora cavernosa (CC) strips from WT, NLRP3^-/- and caspase^-/- mice in the presence of ET-1 (100 nM) and vehicle, MCC950, tiron, BAPTA AM, BQ123, or BQ788. ET-1 reduced the ICP/MAP in WT mice, and MCC950 prevented the ET-1 effect. ET-1 decreased CC ACh-, sodium nitroprusside (SNP)-induced relaxation, and increased caspase-1 expression. BQ123 an ET_A receptor antagon

SUBMITTER: Sobrano Fais R 

PROVIDER: S-EPMC9806301 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature