Eltrombopag increases the hematopoietic supporting ability of mesenchymal stem/stromal cells.

ABSTRACT:

Background

Eltrombopag (EP) is a small molecule that acts directly on hematopoietic stem cells (HSCs) and megakaryocytes to stimulate the hematopoietic process. Mesenchymal stem/stromal cells (MSCs) are key hematopoietic niche regulators.

Objectives

We aimed to determine whether EP has any effect on MSC function and properties (especially on their hematopoietic-supporting ability) and if so, what changes (e.g. genome-wide transcriptomic alterations) are induced in MSC after EP treatment.

Design/methods

MSCs were isolated from 12 healthy donors and treated with 15 µM and 50 µM of EP for 24 h. The toxicity of the drug on MSCs and their differentiation ability were analyzed, as well as the transcriptomic profile, reactive oxygen species (ROS) and DNA damage and the chang

SUBMITTER: Muntion S 

PROVIDER: S-EPMC9806379 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature