Targeted protein degradation by Trim-Away using cell resealing coupled with microscopic image-based quantitative analysis.

ABSTRACT: "Trim-Away" technology enables rapid degradation of endogenous proteins without prior modification of protein-coding genes or mRNAs through delivery of antibodies that target proteins of interest. Although this approach can be readily applied to almost any cytosolic protein, strategies for cytosolic antibody delivery have been limited to microinjection or electroporation, which require skill-dependent operation or specialized equipment. Thus, the development of antibody delivery methods that are convenient, scalable, and preferably do not require detachment of adherent cells is required to extend the versatility of the Trim-Away method. Here, we developed a cell resealing technique optimized for Trim-Away degradation, which uses the pore-forming toxin streptolysin O (SLO) to permeabilize t

SUBMITTER: Kunishige R 

PROVIDER: S-EPMC9806799 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature