Effects of Stromal Cell Conditioned Medium and Antipurinergic Treatment on Macrophage Phenotype.

ABSTRACT: The immunomodulatory capacity of the human mesenchymal stromal cell (MSC) secretome has been a critical driver for the development of cell-free MSC products, such as conditioned medium (CM), for regenerative medicine applications. This is particularly true as cell-free MSC products present several advantages over direct autologous or allogeneic MSC delivery with respect to safety, manufacturability, and defined potency. Recently, significant effort has been placed into creating novel MSC CM formulations with an immunomodulatory capacity tailored for specific regenerative contexts. For instance, the immunoregulatory nature of MSC CM has previously been tuned through a number of cytokine-priming strategies. Herein, we propose an alternate method to tailor the immunomodulatory "phenotype" of

SUBMITTER: Arabiyat AS 

PROVIDER: S-EPMC9807257 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature