Induction of potent antitumor immunity by intradermal DNA injection using a novel needle-free pyro-drive jet injector.

ABSTRACT: The current success of mRNA vaccines against COVID-19 has highlighted the effectiveness of mRNA and DNA vaccinations. Recently, we demonstrated that a novel needle-free pyro-drive jet injector (PJI) effectively delivers plasmid DNA into the skin, resulting in protein expression higher than that achieved with a needle syringe. Here, we used ovalbumin (OVA) as a model antigen to investigate the potential of the PJI for vaccination against cancers. Intradermal injection of OVA-expression plasmid DNA into mice using the PJI, but not a needle syringe, rapidly and greatly augmented OVA-specific CD8⁺ T-cell expansion in lymph node cells. Increased mRNA expression of both interferon-γ and interleukin-4 and an enhanced proliferative response of OVA-specific CD8⁺ T cells, with

SUBMITTER: Inoue S 

PROVIDER: S-EPMC9807518 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature