Transcriptional regulation of Notch1 by nuclear factor-κB during T cell activation.

ABSTRACT: Notch1 plays important roles in T cell development and is highly expressed in activated CD4⁺ T cells. However, the underlying mechanism of Notch1 transcription in T cells has not been fully characterized. Therefore, we aimed to determine how Notch1 expression is regulated during the activation of CD4⁺ T cells. Both the surface expression and mRNA transcription of Notch1 were significantly higher in activated CD4⁺ T cells, but the inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002 or deletion of the Pdk1 gene impaired this upregulation of Notch1. Interrogation of the Notch1 promoter region using serially deleted Notch1 promoter reporters revealed that the - 300 to - 270 region is crucial for its transcription in activated T cells. In addition, we

SUBMITTER: Hwang JR 

PROVIDER: S-EPMC9807580 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature