Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption.

ABSTRACT: Nucleic acid based molecular technologies are the most promising tools for the early diagnosis of Candida infection. A simple and effective DNA preparation method is of critical for standardizing and applying molecular diagnostics in clinic laboratories. The goal of this study was to develop a Candida DNA preparation method that was quick to do, easy to perform, and bio-safe. Snailase and lyticase were screened and combined in this work to enhance the lysis of Candida cells. The lysis solution composition and metal bath were optimized to boost amplification efficiency and biosafety. A duplex real-time PCR was established to evaluate the sensitivity and specificity of the preparation method. Using the supernatant from the rapid preparation method as templates, the duplex PCR sensitivities f

SUBMITTER: He Z 

PROVIDER: S-EPMC9807692 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature