Determination of immunogenic epitopes in major house dust mite allergen, Der p 2, via nanoallergens.

ABSTRACT:

Background

Despite the high prevalence of allergic asthma, currently, avoidance of the responsible allergens, which is nearly impossible for allergens such as house dust mite (HDM), remains among the most effective treatment. Consequently, determination of the immunogenic epitopes of allergens will aid in developing a better understanding of the condition for diagnostic and therapeutic purposes. Current methods of epitope identification, however, only evaluate immunoglobulin E-epitope binding interactions, which is not directly related to epitope immunogenicity.

Objective

To determine and rank the immunogenicity of the epitopes of major HDM allergen, Der p 2.

Methods

We performed degranulation assays with RBL-SX38 cells primed using patient plasma and challenged with

SUBMITTER: Sjoerdsma J 

PROVIDER: S-EPMC9808607 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature