Methyl-SNP-seq reveals dual readouts of methylome and variome at molecule resolution while enabling target enrichment.

ABSTRACT: Covalent modifications of genomic DNA are crucial for most organisms to survive. Amplicon-based high-throughput sequencing technologies erase all DNA modifications to retain only sequence information for the four canonical nucleobases, necessitating specialized technologies for ascertaining epigenetic information. To also capture base modification information, we developed Methyl-SNP-seq, a technology that takes advantage of the complementarity of the double helix to extract the methylation and original sequence information from a single DNA molecule. More specifically, Methyl-SNP-seq uses bisulfite conversion of one of the strands to identify cytosine methylation while retaining the original four-bases sequence information on the other strand. As both strands are locked together to link t

SUBMITTER: Yan B 

PROVIDER: S-EPMC9808626 | biostudies-literature | 2022 Nov-Dec

REPOSITORIES: biostudies-literature