Sequencing Illumina libraries at high accuracy on the ONT MinION using R2C2.

ABSTRACT: High-throughput short-read sequencing has taken on a central role in research and diagnostics. Hundreds of different assays take advantage of Illumina short-read sequencers, the predominant short-read sequencing technology available today. Although other short-read sequencing technologies exist, the ubiquity of Illumina sequencers in sequencing core facilities and the high capital costs of these technologies have limited their adoption. Among a new generation of sequencing technologies, Oxford Nanopore Technologies (ONT) holds a unique position because the ONT MinION, an error-prone long-read sequencer, is associated with little to no capital cost. Here we show that we can make short-read Illumina libraries compatible with the ONT MinION by using the rolling circle to concatemeric consensu

SUBMITTER: Zee A 

PROVIDER: S-EPMC9808628 | biostudies-literature | 2022 Nov-Dec

REPOSITORIES: biostudies-literature