Improving Lentiviral Transduction of Human Adipose-Derived Mesenchymal Stem Cells.

ABSTRACT: Lentiviral transduction of human mesenchymal stem cells (MSCs) induces long-term transgene expression and holds great promise for multiple gene therapy applications. Polybrene is the most commonly used reagent to improve viral gene transfer efficiency in laboratory research; however, it is not approved for human use and has also been shown to impair MSC proliferation and differentiation. Therefore, there is a need for optimized transduction protocols that can also be adapted to clinical settings. LentiBOOST (LB) and protamine sulfate are alternative transduction enhancers (TEs) that can be manufactured to current Good Manufacturing Practice standards, are easily applied to existing protocols, and have been previously studied for the transduction of human CD34⁺ hematopoietic stem

SUBMITTER: Collon K 

PROVIDER: S-EPMC9808795 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature